Study on Quality Standard of Tricyrtis maculata / 中国药房

OBJECTIVE： To establish the quality standard of Tricyrtis maculata. METHODS： The character and microstructure of T. maculata were identified according to the method stated in 2015 edition of Chinese Pharmacopoeia. The contents of moisture， ash and extract were determined. TLC was used for qualitative identification of quercetin and nicotifiorin in samples. The contents of puerarin， p-hydroxybenzoic acid， nicotiflorin， ferulic acid， quercetin and kaempferol were determined by HPLC. RESULTS： The characters and microscopic identification had specificity. TLC spots of quercetin and nicotifiorin were clear and well-separated without interference from negative control. The water content， total ash content， acid insoluble ash content， alcohol soluble extract and water soluble extract for 10 batches of samples were 0.61％-1.89％，6.28％-8.88％，0.76％-1.79％，1.31％-2.00％ and 9.39％-14.27％， respectively. The linear range of puerarin， p-hydroxybenzoic acid， nicotiflorin， ferulic acid， quercetin and kaeniphenol were 0.031 92-0.111 7 μg （r＝0.999 6）， 0.085 3-0.298 5 μg （r＝0.999 5）， 0.010 76-0.037 66 μg （r＝0.999 8）， 0.070 08- 0.245 3 μg （r＝0.999 8），0.058 56-0.205 0 μg （r＝0.999 4），0.009 860-0.033 88 μg （r＝0.999 4）， respectively. The quantitation limits were 1.06， 0.47， 0.75， 1.40， 1.20， 0.74 ng， and the detection limits were 0.36， 0.12， 0.30， 0.53， 0.60， 0.31 ng， respectively. RSDs of precision， stability and repeatability tests were all less than 2％. The average recoveries were 101.54％， 102.10％， 101.46％， 103.35％， 99.36％ and 96.85％， respectively； RSDs were 1.76％， 1.68％， 1.56％， 1.26％， 0.91％ and 1.96％， respectively （n＝6）； the results of the content were 0.017-0.047， 0.042-0.140， 0.003 8-0.015 0， 0.049-0.180， 0.024- 0.091， 0.003 9-0.011 0 mg/g. CONCLUSIONS： The established quality standard can be used for the quality control of T. maculata.

Effect of total knee arthroplasty on joint function and the inflammatory factor of the patients with severe knee osteoarthritis / 国际外科学杂志

Objective To observe and evaluate the mechanism and clinical values of Total Knee Arthroplasty in treating the patients with severe knee osteoarthritis.Method To review and analyse 74 patients with severe knee osteoarthritis who were treated in Suzhou Atliliated Hospital of Nanjing Medical College,including 34 male patients and 40 female patients with average aged (58.22 ± 3.24)ranging from 47 to 75.All the patients received TKA.Compared the HSS score,WOMAC and content of TNF-α and IL-1β in knee synovial fluid 3 months before the operation,3 months after the operation and 6 months after the operation.SPSS20.0 software was used for statistical analysis,and ANOVA or nonparametric test was used for comparison among groups of data.Results HSS scores measured at 3 months after the operation [(58.30 ± 6.33)vs (31.31 ± 15.92)] and 6 months after the operation[(72.39 ± 6.68) vs (31.31 ± 15.92)] were better than those measured before the operation,which proved a statistical difference (P ＜ 0.05).WOMAC measured 3 months after the operation and 6 months after the operation were better than those measured before the operation [(31.068 ± 6.771) vs (67.014 ± 7.699);(24.216 ±5.224) vs (67.014 ±7.699),all P ＜0.05].TNF-α in knee synovial fluid measured 3 months after the operation and 6 months after the operation were lower than those measured before the operation [(56.182 ± 7.056) pg/ml vs (85.065 ± 6.666) pg/ml;(30.387 ± 4.194) pg/ml vs (85.065 ± 6.666) pg/ml;all P ＜ 0.05].IL-1β measured at 3 months after the operation and 6 months after the operation were lower than those measured before the operation [(21.411 ± 4.751) pg/ml vs (60.305 ± 6.932) pg/ml;(11.578 ± 4.142) pg/ml vs (60.305 ± 6.932) pg/ml;P ＜ 0.05].Compared with the data at 3 months after the operation,the content of TNF-α [(30.387 ±4.194)pg/ml vs (56.182 ±7.056) pg/mll and IL-1β[(11.578 ±4.142) pg/ml vs (21.411 ± 4.751)pg/ml] were both lower,which proved a statistical difference (P ＜ 0.05).Conclusion TKA can imporve the joint functions and activities of the patients with severe knee osteoarthritis and lower the the inflammatory factor like TNF-α and IL-1β in knee synovial fluid to relief the pain of the patiences and accelerate the process of recovery.

Effect of total knee arthroplasty on joint function and the inflammatory factor of the patients with severe knee osteoarthritis / 国际外科学杂志

Objective@#To observe and evaluate the mechanism and clinical values of Total Knee Arthroplasty in treating the patients with severe knee osteoarthritis.@*Method@#To review and analyse 74 patients with severe knee osteoarthritis who were treated in Suzhou Atliliated Hospital of Nanjing Medical College, including 34 male patients and 40 female patients with average aged (58.22±3.24)ranging from 47 to 75. All the patients received TKA. Compared the HSS score, WOMAC and content of TNF-α and IL-1β in knee synovial fluid 3 months before the operation, 3 months after the operation and 6 months after the operation. SPSS20.0 software was used for statistical analysis, and ANOVA or nonparametric test was used for comparison among groups of data.@*Results@#HSS scores measured at 3 months after the operation[(58.30±6.33)vs (31.31±15.92)] and 6 months after the operation[(72.39±6.68)vs (31.31±15.92)] were better than those measured before the operation, which proved a statistical difference (P<0.05). WOMAC measured 3 months after the operation and 6 months after the operation were better than those measured before the operation[(31.068±6.771)vs (67.014±7.699); (24.216±5.224) vs (67.014±7.699), all P<0.05]. TNF-α in knee synovial fluid measured 3 months after the operation and 6 months after the operation were lower than those measured before the operation [(56.182±7.056)pg/ml vs(85.065±6.666)pg/ml; (30.387±4.194)pg/ml vs (85.065±6.666)pg/ml; all P<0.05]. IL-1β measured at 3 months after the operation and 6 months after the operation were lower than those measured before the operation [(21.411±4.751) pg/ml vs (60.305±6.932)pg/ml; (11.578±4.142) pg/ml vs (60.305±6.932) pg/ml; P<0.05]. Compared with the data at 3 months after the operation, the content of TNF-α [(30.387±4.194)pg/ml vs (56.182±7.056)pg/ml] and IL-1β[(11.578±4.142)pg/ml vs (21.411±4.751)pg/ml] were both lower, which proved a statistical difference (P<0.05).@*Conclusion@#TKA can imporve the joint functions and activities of the patients with severe knee osteoarthritis and lower the the inflammatory factor like TNF-α and IL-1β in knee synovial fluid to relief the pain of the patiences and accelerate the process of recovery.

Clinical effect of Yiqiwenyang Huoxuelishui prescription on patients with cardiorenal syndrome and its influence on gut flora / 中国中西医结合急救杂志

Objective To observe the clinical therapeutic effect of Yiqiwenyang Huoxuelishui [a traditional Chinese medicine (TCM) prescription] on patients with cardiorenal syndrome (CRS) and its influence on gut flora. Methods Sixty-nine patients with definite diagnosis of CRS accompanied by heart-kidney yang deficiency admitted to Department of Geriatrics of the Third People's Hospital of Hangzhou from January 2015 to December 2016 were enrolled, and the patients were divided into two groups according to whether they received traditional Chinese medicine (TCM) or not. The control group (33 cases) was treated with only western medicine; the combination group (32 cases) was treated with conventional western medicine in addition of 100 mL of Yiqiwenyang Huoxuelishui decoction oral administration, twice a day, and the therapeutic course of both groups was 8 weeks. Before and after treatment, the level changes of left ventricular ejection fraction (LVEF), plasma N-terminal pro-brain natriuretic peptide (NT-proBNP), carbohydrate antigen-125 (CA-125), growth differentiation factor-15 (GDF-15), growth differentiation factor-11 (GDF-11), blood creatinine (SCr), blood urea nitrogen (BUN), gut flora changes and the incidence of adverse reactions were observed in the two groups; after treatment, the clinical therapeutic effect of the two groups were compared. Results After treatment, the levels of LVEF and bifidobacteria, lactobacilli in intestinal tract of the two groups were significantly higher than those before treatment; while the levels of NT-proBNP, DGF-15, GDF-11, CA-125, SCr, BUN and enterococcus and enterobacter in the two groups were significantly lower than those before treatment, and the changes of the above indicators in combination group were more obvious than those in the control group [LVEF: 0.51±0.10 vs. 0.46±0.08, NT-proBNP (ng/L): 658±45 vs. 814±57, GDF-15 (ng/L): 604±32 vs. 708±36, CA-125 (U/L): 4.50±0.33 vs. 5.10±0.37, GDF-11 (ng/L): 491±19 vs. 594±23, SCr (μmol/L): 105±11 vs. 125±13, BUN (mmol/L): 8.1±3.5 vs. 9.7±4.2, bifidobacteria (cfu/g): 8.23±0.46 vs. 7.54±0.33, lactobacilli (cfu/g): 7.96±0.31 vs. 7.09±0.23, enterococcus (cfu/g): 6.32±0.19 vs. 7.15±0.28, enterobacter (cfu/g): 6.13±0.33 vs. 7.18±0.37, all P < 0.05]. In the combination group, there were 2 cases with mild abdominal distension and nausea at the initial stage, and 1 case with mild diarrhea, and because the manifestations were so mild, it was not necessary to stop the treatment. No hypotension occurred in both groups, and no liver or kidney function damage was observed. The rate of total therapeutic effect in the combination group was higher than that of the control group [87.5% (28/32) vs. 60.6% (20/33), P < 0.05]. Conclusion Yiqiwenyang Huoxuelishui prescription for treatment of patients with CRS accompanied by heart-kidney yang deficiency was safe and effective, it can elevate the clinical therapeutic effect, quality of life and improve the gut flora.

Cortical bone channel technique for pedicle screw placement / 国际外科学杂志

Over recent years,pedicle screw fixation is widely used in spine surgery,which allows 3-dimensional fixation with a more rigidconstruct and permits a shorter fusion length.However,its fixationeffect is not so good for patients with severe osteoporosis.Cortical bone trajectory screw insertion has been advocated because it is reportedly less invasive,improvesscrew-bone purchase and reduces neurovascular injury.Based on the present evidence,the author evaluates the relative advantages of cortical bone channels.To this end,a search of relevant published studies reporting biomechanical,morphemetricor clinical outcomes after using of cortical bone trajectory screws in patients with spinal pathologies is reviewed.

Clinical experience of primary neurogenic tumors in mediastinum with surgical treatment in 131 cases / 复旦学报（医学版）

Objective To analyze the clinical features,methods of treatment and prognosis of primary neurogenic tumors of mediastinum in patients taking surgical intervention.Methods A database was maintained retrospectively of all patients undergoing surgery for tumor and pathologically diagnosed with primary neurogenic tumors of mediastinum,managed in the Department of Thoracic Surgery,Zhongshan Hospital,Fudan University,Shanghai between Jan.,2008 and Dec.,2014.This work analyzed retrospectively the information about clinical and imaging features,surgical techniques and outcome extracted from medical records.Results Among the 131 cases,78 cases (59.5％) were males,53 cases (40.5％) were females;72 cases were diagnosed incidentally (55.0％),while the other 59 cases (45.0 ％) suffered from different symptoms.The posterior mediastinum was the most principal location with 61 cases in the left and 69 cases in the right,and 1 case remained in the anterior mediastinum.Total 98 cases (74.8％) underwent surgeries via video-assisted thoracic surgery (VATS),5 cases (3.8％) took VATS surgery with small incision,and 28 cases (21.4％) experienced open thoracotomy,with no mortality during perioperative period.Gross total resection was obtained in 130 patients (99.2％).The remaining patient underwent a palliative resection for malignant schwannomas.Of the patients,98 cases had benign schwannomas (74.8％),24 cases had gangliocytomas (18.3％),2 cases had malignant schwannomas (1.5％),2 cases had neurofibromas (1.5％),2 cases had paragangliomas (1.5％),2 cases hadprimitive neurotodermal tumor (PNET) (1.5％) and 1 case had neuroblastomas (0.8％).All patients were followed up from 12 to 95 months with an average of 53 months.A patient with PNET died of tumor metastasis,a patient with malignant schwannomas died after palliative ectomy,and 2 cases died of other reasons.The rest survived until Jan.,2016 with tumor free.Conclusions Nearly no specific clinical symptoms occur in neurogenic tumors of mediastinum.Most of neurogenic tumors of mediastinum are benign with optimistic prognosis after surgical treatment.While malignant neurogenic tumorsusually come with poor prognosis,which places special emphasis on early diagnose together with surgical treatment.

Dynamic expression and mechanism of γδ T cells in the spleen of mouse with experimental autoimmune uveitis / 中华实验眼科杂志

Background Previous studies showed that the pathogenesis of uveitis is related to γδ T cells.However,it remains unclear that how these cells are involved in experimental autoimmune uveitis (EAU).Objective This study aimed to observe the dynamic changes of γδ T cells in EAU and explore the role of γδ T cells in the pathological process of EAU.Methods Forty-five C57BL/6(B6) mice were assigned to the normal control group (six mice) and EAU model group (thirty-nine mice).The mice were immunized subcutaneously at 6 spots on the footpads,tail base,and flank with emulsion containing human interphotoreceptor retinoid binding protein1-20 (IRBP1-20) emulsified in complete Freund's adjuvant.After immunization,the mice were examined for clinical signs of EAU by using a Genesis-D camera.The changes of histopathology were compared by hematoxylin and eosin staining.Mouse lymphocytes were isolated and purified from the spleens of IRBP1-20-immunized or normal B6 mice by using a γδ T-cell isolation kit.Flow cytometry was used to detect the changes of intracellular expression of interleukin-17A (IL-17A),and then transferred the activated γδ T cells into EAU models to analyze the changes of clinical signs and histopathology of EAU.Experimental study program as well as the use and feeding of the animals were authorized by the Animal Management and Use Committee of Shandong Traditional Chinese Medicine University.Results The inflammatory symptoms in mouse eyes appeared on day 12 after modeling.The initial changes were fundal blood vessel thickening and minimal inflammatory cell infiltration.Then,multifocal chorioretinal lesions,serious vasculitis and linear lesions were observed on days 16-20,along with abundant lymphocyte infiltration in the vitreous and retinal disorganization.The inflammation symptom scores and the pathological inflammation scores at different time points after modeling had statistically significant differences (F =51.399,P =0.000;F =47.342,P =0.000).The inflammatory symptoms in the eyes began to abate from day 28 onwards.The number of γδ T cells was obviously increased during the inflammation phase of EAU at day 16-20 after modeling,with the number of γδ T cells was (5.67 ±-0.49) ％ and (5.78 ±±0.55) ％,respectively,which was significantly higher than (1.53 ± 0.14) ％ before modeling,with significant differences between them (both at P＜0.05),meanwhile CD69 levels and the integrin lymphocyte function-associated antigen-1 (LFA-1) and secreted IL-17A were elavated.The secretion level of IL-17A was (13.40±0.50)％ and (17.80±2.37)％ on day 16 and day 20 after modeling,respectively,which was significantly higher than (1.53 ± 0.19) ％ before modeling,with significant differences between them (P =0.000,0.001).The activated γδ T cells were transferred into EAU model,the inflammation symptom scores were 1.00 (1.00,2.00) after activated γδ T cells were transferred into EAU model,which was significantly higher than 0.75 (0.05,1.00) of the untransferred group (Z =27.00,P =0.03),and the symptoms of EAU were aggravated.Conclusions The proportion of γδ T cells reaches peak in inflammation of EAU,and the cells are activated.The activated γδ T cells in the EAU model play a immune regulation role by secreting IL-17A.

Effect of Combined Acupuncture-medication Anesthesia on Visceral Function in Laparoscopic Cholecystectomy / 上海针灸杂志

ObjectiveTo observe the effect of combined acupuncture-medication anesthesia on visceral function of patients undergone laparoscopic cholecystectomy, and to furtherexplore its clinical and theoretical significance.MethodSixty patients who were going to receive laparoscopic cholecystectomy were enrolled and randomized into an observation group (combined acupuncture-medication anesthesia) and a control group (conventional general anesthesia) by the random number table, 30 subjects in each group. The total bilirubin (TBIL), alanine aminotransferase (ALT), aspertate aminotransferase (AST), urea nitrogen, creatinine, and Mini-Mental State Examination (MMSE) were observed to evaluate the effect of combined acupuncture-medication anesthesia on visceral function of patients undergone laparoscopic cholecystectomy.ResultBefore operation, there were no significant differences in TBIL, ALT, urea nitrogen, creatinine, and MMSE between the observation group and the control group (P>0.05), while the AST level of the observation group was significantly higher than that of the control group before operation(P0.05); the TBIL level in the observation group was significantly lower than that in the control group (P0.05). The MMSE score of the observation group was significantly higher than that of the control group 6 h after the operation (P0.05).ConclusionThe combined acupuncture-medication anesthesia and conventional general anesthesia play equal effects on liver and kidney functions in laparoscopic cholecystectomy, thus the two anesthesia methods are both safe andeffective. The combined acupuncture-medication anesthesia has certain protective effects on brain function in early stage, and possibly produces certain protective effectson liver function.

The influence of precise liver resection techniques on intestinal permeability in the diseases needing liver resection / 中国医师杂志

Objective To evaluate the influence of precise liver resection techniques on intestinal permeability in the diseases needing liver resection.Methods Eighty-seven patients needing liver resection were randomized to receive the precise liver resection (n =58,PLR group) or the conventional liver resection (n =29,CLR group).D-lactate and and endotoxin in abdominal fluid were detected in all the patients.The abdominal fluid bacteria cultures were performed.Results The postoperative hospitalization time,the needed time of blood routine,temperature,and oppetite resuming were significantly shorter in PLR group than those in CLR group (t ≥7.36,P ＜ 0.01) ; The postoperative abdominal effusion was significantly less in PLR group than that of CLR group(t ≥ 14.17,P ＜0.01).The abdominal fluid concentrations of D-lactate and endotoxin in operation or at 1d after operation were significantly higher than those at 5d after operation in both groups(t ≥10.41,P ＜0.01).Those parameters decreased significantly at 2d after operation,returned to the normal level at 3d after operation in PLR group,and those parameters were significantly lower in PLR group than those in CLR group at the same time phase after postoperative 2 or 3 days (t ≥9.23,P ＜0.01) ; Those parameters began to drop at 3d after operation,returned to the normal level at 5d after operation in CLR group.The positive rate of abdominal fluid bacteria cultures was significantly lower in PLR group than that in CLR group(13/29) (x2 =23.51,P ＜ 0.01).Conclusions The precise liver resection techniques had an important influence on intestinal permeability in the diseases needing liver resection.

Effects of hippocampal neurogenesis in spatial learning and memory in rats / 中华神经科杂志

Objective To investigate the effects of hippocampal neurogenesis in spatial learning and memory,especially in spatial long-term memory.Methods Totally 24 wistar rats were randomly divided into two groups:normal control group (NC group,n =12) and irradiation group (IRR group,n =12).We use low-dose irradiation at subgranular zone to inhibit adult hippocampal neurogenesis and new neurons were investigated by 5-Bromo-2-deoxyUridine/neuron specific nuclear protein double-labeling.Four weeks after irradiation,Morris water maze,including navigation test and space exploration assay,was used to detect spatial leaming and memory.Then,in the day 8 and day 15 after the navigation test,space exploration assay was repeated to detect spatial long-term memory.The expression levels of postsynaptic density protein 95 and synaptophysin were evaluated using western blot and real-time quantitative PCR.Results Hippocampal neurogenesis was inhibited by low dose irradiation(2.80 ± 0.44 vs 23.50 ± 1.12,t =20.21,P ＜ 0.01).After the inhibition,the escape latency did not change,while the time spent in target quadrant was significantly declined in the day 2((14.76 ±.2.04) s vs (20.47 ± 1.29) s),day 8((11.95 ± 1.34) s vs (19.52±1.43) s)and day 15((11.79 ±1.35) s vs (21.58 ±1.07) s) after the navigation test (t=2.45,P＜0.05; t =3.76,P＜0.01; t =5.39,P＜0.01).The postsynaptic density protein 95 and synaptophysin expressions were significantly decreased in IRR group.As to the RNA levels,there was also a significant difference between the two groups.Conclusion Hippocampal neurogenesis in the dentate gyrus plays an important role for the formation of spatial long-term memory.

Efficacies of sleeve gastrectomy and Roux-en-Y gastric bypass for the treatment of type 2 diabetes mellitus: a Meta-analysis / 中华消化外科杂志

Objective To review the efficacies of sleeve gastrectomy and Roux-en-Y gastric bypass for the treatment of type 2 diabetes mellitus.Methods The Cochrane library,PubMed,China Journal Full Text Database,Chinese Scientific Journal Full Text Database,Chinese Biomedical Literature Database and Wanfang Database were searched with key words including“减重手术”,“胃切除术”,“胃绕道术”,“胃旁路手术”,“胃转流术”,“胃袖状切除术”,“糖尿病”,“bariatric surgery”,“gastric bypass”,“sleeve gastrectomy”,“diabetes,T2DM and et al.Literatures published between the time of database establishment and December 2012 were searched.Literatures focused on the comparison of the efficacies of sleeve gastrectomy and Roux-en-Y gastric bypass for the treatment of type 2 diabetes mellitus were retrieved.Data extraction and quality evaluation of the literatures were done by 2 researchers independently.Meta analysis was performed by RevMan 5.1.2 software.The count data were presented by risk ratio (RR) or odds ratio (OR),and the measurement data were presented by mean difference (MD) or standard mean difference (SMD).The heterogeneity of the literatures was analyzed by I2.Results Five literatures were retrieved,including 164 patients in the sleeve gastrectomy group and 184 patients in the Roux-en-Y gastric bypass group.The results of Meta analysis showed that Roux-en-Y gastric bypass could significantly increase the remission rate of type 2 diabetes (OR =0.48,95 ％ confidence interval:0.26-0.91,P ＜ 0.05),increase the percentage of patients without drugs during follow up (OR =0.37,95 ％ confidence interval:0.16-0.84,P ＜0.05),decrease the levels of HbA1c and body weight (MD =0.28,-0.44,95％ confidence interval:0.14-0.43,-0.76--0.13,P ＜ 0.05) when compared with sleeve gastrectomy.There was no significant difference in the incidence of complications between the 2 groups (OR =1.81,95％ confidence interval:0.20-16.73,P ＞ 0.05).Conclusion Roux-en-Y gastric bypass is superior to sleeve gastrectomy for patients with type 2 diabetes.

Safety and feasibility of fast track surgery in operations for gastric cancer: a meta analysis / 中华消化外科杂志

Objective To estimate the safety and feasibility of fast track surgery (FTS) in operations for gastric cancer.Methods The Cochrane library,PubMed,EMBASE,Chinese biomedical literature database,China journal full text database,Chinese scientific journals full text database were searched with the key words of gastric cancer,gastric carcinoma, FTS,fast track surgery, enhanced recovery after surgery,ERAS, 胃癌,and 快速康复外科.The search was finished in April,2011.Randomized controlled trials of FTS for patients with gastric cancer were included.Two reviewers evaluated the quality of the included studies and extracted the data independently.Meta analysis was performed by RevMan 5.1.2 software.Results Fourteen randomized controlled trials involving 1270 patients were included.The results of meta analysis showed that FTS could significantly shorten the first exhaust time (MD =-6.15,95 ％ confidence interval:-8.22- -4.07,P＜ 0.05) and hospital stay ( MD =- 2.68,95％ confidence interval:- 3.26- - 2.10,P ＜ 0.05 ),and reduced the hospital fees ( MD =- 0.39,95 ％ confidence interval:- 0.55- - 0.22,P ＜ 0.05 ).FTS could lighten the postoperative stress and relatively enhance the immunity.Conclusion Compared with conventional methods,FTS could speed up the rehabilitation for patients with gastric cancer postoperatively without increasing the adverse reactions.

Safety and stability of posterior chamber phakic intraocular lens for correction of extreme myopia: A follow-up of 11 cases / 中国组织工程研究

BACKGROUND: The phakic intraocular lens implantation has advantages of reversibility and accommodative capability, which is a promising refractive operation. Posterior chamber phakic intraocular lens implantation is a developing method for the correction of extreme myopia. OBJECTIVE: To evaluate the efficacy, safety and stability of posterior chamber phakic intraocular lens implantation for the correction of extreme myopia. METHODS: This study included 18 eyes of 11 patients whose received posterior chamber phakic intraocular lens implantation at Shierming Eye Hospital from March 2006 to January 2009, including 4 males (7 eyes) and 7 females (11 eyes). All patients were followed-up 3-6 months, mean 5.3 months. The refraction, intraocular pressure, contrast sensitivity, glare sensitivity, endothelial cell morphometry, as well as lens opacities were observed. RESULTS AND CONCLUSION: After operation, naked visions of the 18 eyes were better than or equal to preoperative corrective vision, and the astigmatism was relieved obviously. The naked contrast sensitivity and glare sensitivity was better than that before the operation. The anterior chamber depth, intraocular pressure and corneal endothelium counting before and after the operation had no significant difference. No serious complication was detected during the follow-up. The vision decent at night and dazzling sensation occurred in 2 cases. Shot-term observation shows that implanting intraocular lens in posterior chamber to correct high myopia is safe, stable and predictable. This operation is a promising method for correcting high myopia.

Eye black immunosuppression decoction in treatment of rejection after corneal transplantation in 26 cases / 中国组织工程研究

Twenty-six eyes which appeared rejection after allograft penetrating keratoplasty between April 2003 and April 2004 in Shandong Shi'erming Eye Hospital were collected, including 16 males and 10 females, 9 cases were repeated relapsing viral keratitis, 8 cases were traumatic posterior corneal leucoma, 5 cases were corneal decompensation caused by various intraocular surgery, 2 cases were congenital leucoma, and 2 cases had other symptom. The rejection occurred at 2 month to 2.5 years after operation. Among them 19 cases were transferred to our hospital and asked adding traditional Chinese therapy because western medicine was ineffective in other hospital. All cases were received local treatment with hormone and non-steroidal anti-inflammatory eyedrop, some cases applied systemic administration with glucocorticoid, mainly based on eye black immunosuppression decoction, potion daily, twice per day, the average time of medication was 11-42 days. Clinical symptoms were served as observation indexes, results showed that the total effective rate of the 26 cases were 100%. The bulbar conjunctiva hyperemia and edema was reduced, corneal opacity became light, and rejection was complete response in 9 cases, 5 of 19 cases which were transferred to our hospital were exempted from operation, and the average vision was improved three lines. It was found that purging liver and eliminating dampness compound Chinese medicine can rapidly reduce anterior chamber's inflammatory response and backoff corneal neovascularization in clinical practice.

A Clinical Study on Gut Barrier Function Change after Acute Intraperitoneal Hemorrhage / 中国医师杂志

12h interval from injury to operation had significantly higher concentration of plasma D-lactate and endotoxin at above metioned 4 time points. Plasma concentration of D-lactate and endotoxin in the last two groups declined to normal range at 72h after operation, while in the first two groups reduced to normal range before leaving hospital. Conclusion Acute intraperitoneal hemorrhage could damage gut barrier function, and injury degree was related with interval from injury to operation and blood loss amount.

Surgical treatment for abdominal aortic aneurysm in 46 cases / 中国普通外科杂志

Objective To study how to improve the safety of the operation for abdominal aortic aneurysm(AAA).Methods Forty-six cases of AAA received resection of AAA plus artificial blood vessel transplantation in recent three and a half years in our 2 hospitals.The lesions involved only the abdominal aorta in 20 cases,and extended to unilateral common iliac and internal and external iliac arteries in 8 cases,to bilateral common iliac and external and internal iliac arteries in 16 cases,and involved the renal artery in 2 cases.Emergency operation was done in 3 cases.Results In these 46 cases,45 were cured and 1 died,and no operative complications occurred.Conclusions Surgical operation is the best way to treatment AAA.